Principal Investigator: Akiko Shimamura, MD, PhD
Professor of Pediatrics, Harvard Medical School
Samuel E. Lux IV Chair in Hematology Oncology
Director, Bone Marrow Failure and MDS Program

Our laboratory is investigating the genetic basis of bone marrow failure and leukemia predisposition, and developing experimental models to gain mechanistic insights into disease pathogenesis and to develop novel therapies. We have identified genes causing marrow failure and leukemia.  We have established a repository of clinically annotated samples that are used by our lab and shared with collaborators worldwide. 

Our laboratory is located in the department of pediatric Hematology/Oncology at Harvard, which offers a vibrant team-based environment to conduct clinically-relevant research using a variety of genomic, molecular, biochemical, and cell-based technologies to model bone marrow failure and leukemia predisposition. Current projects in the lab involve genomic and transcriptomic studies, mammalian cell culture, and functional genomic screening in vitro and in vivo using RNA interference and CRISPR/Cas9. 

Experience with hematopoiesis or computational biology is desirable but not required.  Research techniques include cell culture, plasmid preparation, cloning, qPCR, shRNA and CRISPR design, genomic DNA sequencing, DNA preps from primary patient samples, transfection of cultured cells, western blotting, immunoprecipitation, immunofluorescence, cell survival assays, flow cytometry, methylcellulose colony assays, lentiviral vector production, RNASeq, genomics, and single cell analyses. 

The ideal candidate will have prior experience with laboratory research in molecular biology, molecular cloning, mammalian cell culture, gene transfer, genomics, and transcriptomics. Familiarity with computational biology is advantageous but not required. We are seeking outstanding and highly motivated candidates with strong organizational and time management skills, the ability to work both independently and as part of a team, and strong communication skills.

To inquire about open positions, please send your CV to Kathleen.Zheng@childrens.harvard.edu